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Selecting Ultra-Micro Cuvettes for
Your Spectrophotometer
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For detection, spectrophotometers shine a beam of light through a sample in a cuvette. The height of the beam from
the bottom of the cuvette chamber is termed the “beam height.” Two competing industry “standards” have developed for
the beam height: 8.5 mm and 15 mm.
When working with macro and semimicro cuvettes, the beam height determines only the minimum fill volume; the cuvette
must be filled enough to reach the spectrophotometer beam height. In contrast, the volume of sample in an
ultra-micro cuvette is so small that the sample chamber in the cuvette must be elevated from the bottom of the
cuvette so that the sample lines up with the light beam.
Because of the competing standards for spectrophotometers, ultra-micro cuvettes are made with window heights that
align with either the 8.5 mm or 15 mm beam-heights. It is critical that the window height of your ultra-micro
cuvette matches the beam height of your spectrophotometer. With the wrong window height, the beam either will not
pass through the sample at all, or will need much more than the minimum-specified sample volume to provide a result.
Before selecting an ultra-micro cuvette, you need to know the beam height of your spectrophotometer. If you aren’t
certain of the beam height of your spectrophotometer, check the list below. If your spectrophotometer is not listed,
consult the operating manual of your instrument.
Note: Data in this application were collected from public sources, and are correct to the best of our knowledge at
the time of publication. This is provided as a customer convenience. Manufacturers may choose to offer instruments
conforming to either standard for technical or corporate reasons. BrandTech Scientific does not manufacture
spectrophotometers, and makes no warranty regarding the information provided by this application.
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