Graduated Pipettes – Class A, USP, Certified

Home>Volumetric Labware>Glassware>Graduated Pipettes – Class A, USP, Certified

Graduated Pipettes – Class A, USP, Certified

Product and ordering information for Graduated Pipettes – Class A, USP, Certified. Type 2 total delivery pipette only requires setting meniscus one time. Available in all ISO sizes from 1mL to 10mL.  Supplied with a batch number and an accompanying USP batch certificate in the original packaging.

Catalog #: N/A Category:
   

Description

Class A, USP, certified graduated pipette. Type 2 total delivery pipette only requires setting meniscus one time. In the DIN EN ISO 835 standard, the waiting time has been reduced from 15 to 5 seconds. High contrast blue enamel represents the optimum combination of resistance and readability. Full ring marks at major graduations. Made with BRAND's flexible screen stencils which ensures the volume markings match the calibration marks for enhanced accuracy at all intermediate volumes. Available in all ISO sizes from 1mL to 10mL. Made of AR-GLAS®.

The optimized flow geometry in the tip ensures reliable liquid dispensing from the pipette. The mechanical resilience of the tip and its fire-polished edges ensures a long service life.

All BLAUBRAND® USP graduated pipettes are supplied with a batch number and an accompanying USP batch certificate in the original packaging.

Volumetric instruments according to USP error limits

Companies producing pharmaceutical products for the US market are supervised by US authorities, e.g. the FDA (Food and Drug Administration), and have to meet the requirements of the USP (United States Pharmacopeia). The current USP prescribes in section 31 the use of volumetric instruments with accuracy in accordance to class A of the ASTM standards and list those for volumetric flasks, transfer pipettes and burettes in tables. For graduated pipettes up to and including 10 mL size the error limits are mentioned in the text. BRAND volumetric instruments are manufactured according to the current DIN EN ISO standards. As the construction specifications are differently defined in the DIN EN ISO and ASTM standards, the error limits differ as well. With included USP certificates BRAND confirms for the delivered USP volumetric instruments that the class A error limits according to the USP or, rather, ASTM standards are met.

Every glass volumetric measuring instrument is individually calibrated. Computer-controlled systems ensure maximum precision in a fully automated production line. The final step of this Statistical Process Control is random sampling of finished product according to DIN ISO 3951. The quality management system applied at BRAND, which is certified according to DIN EN ISO 9001, is a combination of process monitoring and random checks with an accepted quality level (AQL) of approximately 0.4.

The batch certificate confirms that ASTM class A error limits required by USP are met. The certificate records the mean value, the standard deviation of the batch and the date of issue (batch number: year of manufacture/batch).

Product No. & Specs



Class A, USP, Certified Graduated Pipettes

  Volume
   mL
Subdivision
mL
Error limits
± mL
Length
mm
Qty/Pack
Cat. No.
  1
0.01
0.01
360
12
27506
  2
0.02
0.01
360
12
27509
  5
0.05
0.02
360
12
27511
  10
0.1
0.03
360
12
27513

FAQs

What is Boro 3.3 glass?

The abbreviation Boro 3.3 stands for a borosilicate glass type 3.3 as specified in international standard DIN ISO 3585, for applications requiring very good chemical and thermal resistance (including resistance to thermal shock), and high mechanical stability. Typical applications are components for chemical apparatus, round-bottom flasks, and beakers.

What is AR-GLAS®?

AR-GLAS® is a type of soda-lime glass with good chemical and physical properties. Soda-lime glass is the most common type (90%) of manufactured glass. It is suitable for products which are usually subjected to short-term chemical exposure, and to limited thermal stress (e.g. pipettes, culture tubes).

What pipette controllers can be used with the bulb and graduated pipettes?

Any of the pipette controllers offered by BrandTech® can be used. Depending on your application, choose the macro™ for manual control or the accu-jet® pro for motorized control. If using the accu-jet® pro, a 3µm membrane filter AND the gravity dispense mode must be used to obtain the proper drain times.

How should I clean my volumetric glassware?

For increased service life it is recommended that you clean your volumetric instruments with:

  • No mechanical abrasion
  • Low alkaline detergents
  • Low temperature: < 70°C
  • Short duration: < 20 minutes

What is the advantage to using glass vs. plastic?

Glass has very good chemical resistance against water, saline solutions, acids, alkalis and organic solvents, and in this respect surpasses the majority of plastics. It is only attacked by hydrofluoric acid, and - at elevated temperatures - by strong alkalis and concentrated phosphoric acid. Further advantages of glass are its dimensional stability, even at elevated temperatures, and its high transparency.

What kind of screening ink is used for printing the volumetric glassware?

Blue and white enamel are printed on the outer surface and provide a high contrast and an optimal combination of chemical resistance and legibility for the volumetric glassware.

What is a Type 2 graduated pipette?

Graduated pipettes are classified by types (1, 2, or 3). The types differ in how they are marked and, therefore, how they are handled:
Type 1: Nominal capacity above the tip and the zero point at top; Set the meniscus at zero point and discharge the liquid to lower volume.
Type 2: Nominal capacity at the top; Set the meniscus for the volume intended to deliver and discharge the liquid completely. This is the only type that requires just one meniscus reading so it is quicker and easier to use, and is the only type that BrandTech® sells.
Type 3: Zero point at top; Set the meniscus at zero point and discharge the liquid partially to one lower volume mark or completely.

What is the proper way to use the pipette?

  1. Fill pipette to approximately 5mm above selected graduation mark.
  2. Remove any liquid remaining on the outside with a tissue.
  3. While holding the pipette vertically, set the meniscus.
  4. Wipe off any drops of liquid.
  5. Align tip at inner surface of inclined vessel.
  6. Deliver liquid and, after waiting time, draw the pipette tip upwards along the inner wall.

There is a small amount of residual liquid in the tip. Should it be blown out?

No. The small amount of residual liquid has been taken into account during calibration ("To deliver" or TD calibration) of the pipette and should not be blown out.

Can you explain the waiting time for pipettes?

The waiting time begins when the meniscus comes to rest at the lower volume mark or in the tip: During the waiting time residual liquid continues to flow down from the glass wall. Meniscus may need to be set once again after waiting time.

How can the meniscus be easily read?

The volume of a concave meniscus has to be read at the lowest point of the liquid level. Here the lowest point of the meniscus has to touch the upper edge of the graduation mark. For parallax-free adjustment of the meniscus, the pipette is held upright and the observer's eye must be at the same height as the meniscus. In this position the ring mark will appear as a line. If the background is light, a piece of dark paper held behind the instrument immediately beneath the ring or graduation mark will make the meniscus appear darker and more easily readable.

Tech Info

Volumetric Glassware SOP

Literature

Catalog Page
BRAND Class A, USP, Volumetric Glassware
Are Your Glass Volumetric Instruments Accurate?
USP Requirements for Volumetric Instruments