BRANDplates® Insert Strips

BRANDplates® Insert Strips

Product and ordering information for BRANDplates® Insert Strips: For use with BRANDplates® Insert System microplates; Smooth-walled or Inlet Opening System; PC or PET; 0.4µm, 1.0µm. 3.0µm 0.8µm; for secretion, chemotaxis, migration, co-culture, etc.

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Description

The BRANDplates® Insert System, co-developed with the Fraunhofer Society, is an innovative microplate system for cell culture applications such as co-culture, migration/invasion assays, and in vitro preparation of 3D tissue models. The system consists of two types of cell culture plates and a selection of cell culture inserts. The 24-well standard model is used in such applications as co-culture, migration/invasion assays, and chemotaxis assays, while the specialized 6-well model is well-suited for the automated in vitro preparation of human tissues (3D tissue culture). Key features to consider when selecting which components to use are plate selection and well size, insert type, and membrane pore size and material.

Inserts are available either smooth-walled or with special inlet channels (the Inlet Opening System*). The smooth-walled inserts are well-suited for standard applications such as secretion studies, co-culture, and chemotaxis studies. The Inlet Opening System enables rapid, consistent changing of media, from submersion culture to air-lift culture. The special inlet channels also enable adjustment of the medium level without damage to the tissue model. Inserts are also available with either PC (translucent) or PET (transparent) membranes for different optical requirements and cell visiblity, and in multiple membrane pore sizes for different applications. Through the many possible combinations of the Insert System you can respond flexibly to the different experimental needs and create perfect culture conditions.

Insert Pore size
Areas of application
0.4µm
Co-culture, transport studies, secretion studies, cell polarity studies, etc.
1.0µm
Co-culture, transport studies, secretion studies, etc.
3.0µm
Migration/invasion studies, chemotaxis studies, metastasis experiments, etc.
8.0µm
Migration/invasion studies, chemotaxis studies, metastasis experiments, etc.

*Patent pending

Product No. & Specs

BRANDplates® Smooth-walled insert strips -
PS, cellGrade™ plus surface, sterile. 12 strips of 4 divisible inserts (Individually wrapped).

  Pore Size (µm)
PC membrane
Cat. No.
PET membrane
Cat. No.
  0.4
782800
782810
  1
782820
-
  3
782840
-
  8
782860
782870


BRANDplates® Strips with Inlet Opening System -
PS, cellGrade™ plus surface, sterile. 12 strips of 4 divisible inserts (Individually wrapped).

  Pore Size (µm)
PC membrane
Cat. No.
PET membrane
Cat. No.
  0.4
782801
782811
  1
782821
-
  3
782841
-
  8
782861
782871

FAQs

Is it possible to divide the insert strip?

Yes, it is possible to break the insert strips into individual inserts.

Is it possible to remove the membrane from the inserts?

Yes. With a sharp blade, the PET and PC membranes can be removed from the insert. PET membranes are slightly more stable than PC membranes and won't curl after they are released from the insert.

What is the distance between the well bottom and the membrane?

There is 4mm between the well bottom and the membrane.

Are the BRANDplates® inserts compatible with 24-well plates of other manufacturers?

No. The extended edge of the insert is precisely matched to the 24- and 6-well BRANDplates®. Closing the gap between insert and well significantly reduces the risk of contamination during media changes.

Do the BRANDplates® inserts have to be refrigerated?

No. BRANDplates® inserts are not biologically coated and therefore can be stored at room temperature protected from sunlight.

What is the Inlet Opening System?

The Inlet Opening System (IOS) is a series of openings in the inserts. The combination of the IOS with modified 6-well BRANDplates® speeds up medium exchange in all wells and inserts of a row. Medium levels in inserts and well equilibrate automatically. During medium exchange the IOS provides an slow and equal flow into the inserts by which sensible monolayers are not disturbed.

Are membranes of BRANDplates® Inserts cellGrade™ plus treated on both sides?

Yes. The insert membrane is treated on the apical as well as basolateral side.

Are cells cultured on PC or PET membrane visible under light microscope?

With the help of phase contrast microscopy, cells cultured on PET-membranes can be seen well. Cells cultured on PC-membranes are poorly visible.

Tech Info

Reconstruction of Human Epidermis using BRANDplates® Insert System
Learn the key steps for reconstruction of human epidermal equivalents, plus learn tips for the handling of the BRANDplates® Insert System.

Reconstruction of Full Thickness Skin Equivalents using BRANDplates® Insert System
Learn the key steps for reconstruction of full thickness skin equivalents, plus learn tips for the handling of the BRANDplates® Insert System.

Literature & Videos

Catalog Page
BRANDplates® Insert System flyer

Sample Request

To request a free sample click here and complete the sample request form.