BRANDplates® Insert System Packages

BRANDplates® Insert System Packages

Product and ordering information for BRANDplates Insert System Packages: Microplates & inserts for co-culture, migration, secretion, chemotaxis, 3D cell culture, etc.; suitable for robotics/automation.

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Description

The BRANDplates® Insert System, co-developed with the Fraunhofer Society, is an innovative microplate system for cell culture applications such as co-culture, migration/invasion assays, and in vitro preparation of 3D tissue models. The system consists of two types of cell culture plates and a selection of cell culture inserts. The 24-well standard model is used in such applications as co-culture, migration/invasion assays, and chemotaxis assays, while the specialized 6-well model is well-suited for the automated in vitro preparation of human tissues (3D tissue culture). Key features to consider when selecting which components to use are plate selection and well size, insert type, and membrane pore size and material.

The BRANDplates® Insert System is available as a pack of 5 plates (incl. lids with condensation rings), plus 30 insert strips. The plates included are the 6-well specialized plates with the cellGrade plus surface treatment. The insert strips are available as smooth-walled or with the Inlet Opening System, PC (translucent) or PET (transparent) membrane, and in multiple pore sizes.

Specialized 6-well Plate: The specialized 6-well plate has 6 rows of 4 wells connected as one large, elongated well. This well is fitted with a strip of 4 inserts so that all 4 of the inserts in the strip can be supplied with medium at the same time. Particularly well-suited to the use of insert strips with inlet channels (IOS).

Insert Pore size
Areas of application
0.4µm
Co-culture, transport studies, secretion studies, cell polarity studies, etc.
1.0µm
Co-culture, transport studies, secretion studies, etc.
3.0µm
Migration/invasion studies, chemotaxis studies, metastasis experiments, etc.
8.0µm
Migration/invasion studies, chemotaxis studies, metastasis experiments, etc.

Product No. & Specs

BRANDplates® Insert System (6-well plates filled with 6 insert strips) - PS, cellGrade™ plus surface, sterile. Smooth-walled strips or strips with Inlet Opening System (IOS). Pack of 5 plates with lids (condensation rings) and 30 insert strips.

Insert System with Smooth-walled Insert Strips
  Pore Size (µm)
PC membrane
Cat. No.
PET membrane
Cat. No.
  0.4
782802
782812
  1
782822
-
  3
782842
-
  8
782862
782872

Insert System with IOS Insert Strips.
  Pore Size (µm)
PC membrane
Cat. No.
PET membrane
Cat. No.
  0.4
782803
782813
  1
782823
-
  3
782843
-
  8
782863
782873

FAQs

What is the advantage of the lateral feeding port?

With the feeding port, the lower reservoir is accessible without moving the inserts allowing for easy media exchanges and suitability for robotics/automation. In addition, mechanical stress on the cells/tissue caused by movement of the inserts is avoided.

What is special about the modified 6-well plate?

The 6-well specialized plate has 4 wells connected as one large, elongated well. It is designed to facilitate media exchange in the lower reservoir simultaneously for 4 inserts in a row.

What is the maximum volume of one well of a 24-well plate?

The maximum volume of one well is 3.1mL.

What is the distance between the well bottom and the membrane?

There is 4mm between the well bottom and the membrane.

Are the BRANDplates® inserts compatible with 24-well plates of other manufacturers?

No. The extended edge of the insert is precisely matched to the 24- and 6-well BRANDplates®. Closing the gap between insert and well significantly reduces the risk of contamination during media changes.

Is it possible to divide the insert strip?

Yes, it is possible to break the insert strips into individual inserts.

Is it possible to remove the membrane from the inserts?

Yes. With a sharp blade, the PET and PC membranes can be removed from the insert. PET membranes are slightly more stable than PC membranes and won't curl after they are released from the insert.

Do the BRANDplates® inserts have to be refrigerated?

No. BRANDplates® inserts are not biologically coated and therefore can be stored at room temperature protected from sunlight.

What is the Inlet Opening System?

The Inlet Opening System (IOS) is a series of openings in the inserts. The combination of the IOS with modified 6-well BRANDplates® speeds up medium exchange in all wells and inserts of a row. Medium levels in inserts and well equilibrate automatically. During medium exchange the IOS provides an slow and equal flow into the inserts by which sensible monolayers are not disturbed.

Are membranes of BRANDplates® Inserts cellGrade™ plus treated on both sides?

Yes. The insert membrane is treated on the apical as well as basolateral side.

Are cells cultured on PC or PET membrane visible under light microscope?

With the help of phase contrast microscopy, cells cultured on PET-membranes can be seen well. Cells cultured on PC-membranes are poorly visible.

Tech Info

Reconstruction of Human Epidermis using BRANDplates® Insert System
Learn the key steps for reconstruction of human epidermal equivalents, plus learn tips for the handling of the BRANDplates® Insert System.

Reconstruction of Full Thickness Skin Equivalents using BRANDplates® Insert System
Learn the key steps for reconstruction of full thickness skin equivalents, plus learn tips for the handling of the BRANDplates® Insert System.

Literature & Videos

Catalog Page
BRANDplates® Insert System flyer

Sample Request

To request a free sample click here and complete the sample request form.